FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6035426
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT12700.014.3419349
CGTTGGA20100.014.1779131
AAACGGG20550.014.1157627
AACGGGA17250.014.0786528
CGGGATT12000.013.8668210
GAAACGG20850.013.2701756
GGAAACG21700.013.0009035
CGCGCCC3253.6379788E-1112.45708233
CCGATTG1506.2906113E-412.0371033
CACCGCG4500.011.99570830
CGCCCGG5650.011.54320335
ACGCCCG3103.6407073E-810.88248234
CGTATGC2301.6999125E-510.78194745
ACCGCGC4850.010.66628631
ACCCGGC4253.45608E-1110.58269636
TTCGAGC1700.001848446610.5819947
CGTAGTA1954.69076E-410.4386831
CCGGCTA5200.010.3798738
TCTCGTA3053.4006916E-710.32323543
CCCCGAT2201.2443651E-410.2805211
Produced by FastQC (version 0.11.5)