FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7240163
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT14600.013.4054579
CGGGATT13000.012.97875710
AACGGGA20150.012.6159118
CGTTGGA22650.012.5388621
AAACGGG24750.011.816357
GCGTGAG17850.011.4688122
GAAACGG25400.011.4287096
ACAGGCG24300.011.20196718
ACGCCCG4355.456968E-1210.86038634
ACCGCGC5500.010.63471431
CGTGAGC18850.010.62169523
CGCATAG2352.1604734E-510.5506841
CGCGCCC3857.203198E-1010.51785133
GGAAACG26750.010.4375175
CGCCCGG6900.010.43316535
CCGGCTA7900.09.96680938
CACCGCG6200.09.79686830
AGGCGTG21650.09.76753920
GGCGTGA21550.09.60407921
CGGCTAA8000.09.56101839
Produced by FastQC (version 0.11.5)