FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11786346
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT24700.013.754479
CGTTGGA38700.013.6926991
GAAACGG40300.013.2317226
AAACGGG40300.013.23144157
AACGGGA33250.013.19492158
GGAAACG42150.012.4374515
CGGGATT24550.012.18883410
CGCCCGG11700.09.80697935
CGGCCGA2150.00120621579.4183239
AACGGGT10100.08.6877458
ACGCCCG6851.8189894E-128.53952534
CGGGATA4752.6697307E-88.52591310
ACTGCGC9950.08.36622531
CGGCTAA11600.08.34026439
CGGGAAT13800.08.31482410
ACCGCGC9200.08.31461231
CCGGCTA11950.08.28426838
GTTGGAA72100.08.271762
CACCGCG9800.08.26470630
ACAGGCG40700.08.18115418
Produced by FastQC (version 0.11.5)