FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9850087
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6100.019.91738344
TCTCGTA6950.017.48144543
TATCTCG7400.017.02638441
TCGTATG7350.016.53007345
GATCGGA7300.015.7286451
ATCTCGT8900.013.90404142
ATCGGAA8950.013.3269032
ACACGTC10050.012.53677713
ACCGCGC7600.012.13776331
CACCGCG8150.011.59465530
TCGGAAG11050.010.9978523
CGTCTGA11350.010.90261716
ACGTCTG12150.010.74028115
CGCGCCC5450.010.73364933
CCGCGCC8550.010.52602632
CGCCCGG10700.010.30345435
CCCGAAA5250.010.291911
CGGAAGA12850.09.8075674
CGTTGGA26250.09.6915481
CACGTCT13750.09.49050314
Produced by FastQC (version 0.11.5)