FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823123
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG105360.37320371801016106TruSeq Adapter, Index 11 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC32710.11586459392665498TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13850.039.63630745
GCGGTAC250.002107961635.99883736
GATCGGA4200.034.3680041
TCTCGTA17200.033.7483140
ATCGGAA17450.033.475571
CGGCTAC17150.033.4525532
CTCGTAT17400.033.23109441
ACGGCTA17250.033.12819331
TCACGGC17500.032.5263729
ACACGTC18000.032.4966512
TCGGAAG18000.032.3785442
ATCTCGT17950.032.33878739
TCGTATG17900.032.30285342
ACGTCTG18400.031.66793314
CGTCTGA18650.031.6053215
GTCACGG17950.031.58560428
AGTCACG17650.031.3576527
CGTATGC18500.031.25463943
CATCTCG18700.031.16209638
CGGAAGA18800.031.0001813
Produced by FastQC (version 0.11.5)