FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553838
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT4200.028.38267134
CCGTACG4450.026.28270333
TCTCGTA5350.022.7019643
CTCGTAT5500.022.49175644
ACCGTAC5250.022.27772132
GTACGTA6450.019.17901835
CACCGTA6450.018.48159831
CGTAATC6600.018.06156238
TCGTATG6750.018.03247545
ATCTCGT6950.017.47560942
GATCGGA6750.017.0435731
TACGTAA7650.016.17054436
GTCACCG7500.015.89417629
ACACGTC7850.015.75843513
ACGGGAT10800.015.4109029
ATCGGAA7900.015.1336272
CGGGATT9850.014.84216310
CGTCTGA8050.014.80812516
TCGGAAG8150.014.6694043
CGTTGGA18750.014.3166011
Produced by FastQC (version 0.11.5)