FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7409805
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC105530.1424194023999282TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15350.034.9779621
CTCGTAT15600.033.1608842
CGTATGC15650.033.05493544
TCTCGTA15750.032.70225541
ATCGGAA16750.032.0544322
TCGTATG16350.031.7770943
CGTCTGA17350.030.46368216
ACACGTC17700.030.3695713
ATCTCGT17150.030.03248240
TCGGAAG18300.029.5859893
CGGAAGA18550.029.3088684
ACGTCTG18400.028.84749815
CACGTCT20050.026.69786614
GCACACG20950.025.87301611
GTATGCC20950.025.17689945
CACACGT22000.024.63816612
GATCTCG21050.024.4682739
GTCACAC28150.018.85604129
AGAGCAC32950.016.6551468
GAGCACA33500.016.515989
Produced by FastQC (version 0.11.5)