FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841469
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA500.002268152822.4958926
CGTTGGA18300.014.1658541
ACGGGAT14000.012.8547969
GAAACGG19750.012.6432616
AACGGGA17650.012.6180938
CGGGATT12250.012.48751610
AAACGGG20350.012.3810327
GGAAACG22750.011.2726665
CGGGATA2751.0018266E-610.63442210
AGGCGCC5650.010.35224420
CCGGCTA6350.010.2771738
CGGCTAA6400.010.19613539
CGTATGC3552.4159817E-810.14247845
ACGCCCG3451.7278762E-79.78425634
CCCGTAA2801.3953415E-59.6609371
CCCGAAA2801.3953415E-59.6609371
CTCGTAT3155.661106E-69.28727743
TCTCGTA3401.4881607E-69.26626542
GGATCGT2952.6386786E-59.15408234
TATCTCG3502.206587E-69.00118640
Produced by FastQC (version 0.11.5)