FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7900532
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3950.022.21435545
TCTCGTA4500.020.00051542
CGTTTCG4500.019.49926832
CTCGTAT4300.019.36096243
TCGTATG5300.016.5570344
TTCGGAA5600.016.4724935
TTTCGGA6050.015.6191534
GTTTCGG5800.015.51675633
ATCTCGT6150.014.2686641
TCGGAAT6450.013.95287636
GTCACGT6650.013.1949128
AGTCACG6800.012.90376427
CGTTGGA24150.012.3020341
ACGCCCG5050.011.5836534
ATCGGAA7600.011.5497131
CGGAATC8500.011.38178237
AACGGGA20800.011.2493648
TCGGAAG7700.011.1074162
CGTCTGA7950.011.03739115
ACGGGAT16000.010.9681299
Produced by FastQC (version 0.11.5)