FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12710769
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA236260.18587388379098072TruSeq Adapter, Index 6 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC213860.1682510318612509TruSeq Adapter, Index 6 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25400.026.4481871
CACGCCA38200.020.37632230
ACACGTC52200.015.05524112
GTCACGC51050.014.98297128
CGTCTGA52600.014.96961715
TCGGAAG53000.014.8565812
ATCGGAA52850.014.8486681
AGTCACG52950.014.50209927
CGGAAGA54500.014.4476853
CACGTCT54750.014.43610413
ACGTCTG54350.014.4048314
GCACACG56050.014.15490510
CACACGT58200.013.52892411
AGAGCAC70550.011.5645517
TCACGCC70200.011.3230229
CAGTCAC69700.011.31826526
TCTGAAC71400.011.19608417
AGCACAC72350.011.1731889
GAAGAGC73800.011.1161085
GAGCACA73450.010.9650198
Produced by FastQC (version 0.11.5)