FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924158
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA1009.0753565E-720.2485286
AACGGGA23000.013.498648
AAACGGG27750.013.377487
CGTTGGA27050.013.2685581
GAAACGG28250.012.5831566
ACGGGAT16950.012.0784299
GGAAACG29550.012.0295145
CGTCCCG1558.5089996E-411.61179735
CCGTCCG1802.3491979E-411.2491195
CGGGATT15500.010.45050110
GTCCGAA2201.312509E-410.2265867
CGGGAAT9900.010.22618610
CGTCAAT3309.505475E-79.5765261
CTCGTAT2658.2528946E-59.33873544
CGCGCCC5659.094947E-129.15826633
ACGGGAA10650.09.0835919
ACGGGTT5251.9645086E-108.9990949
CGGGATA3807.039325E-78.88063410
AACGGGT5555.857146E-108.5126568
CCGAGTA15700.08.4650442
Produced by FastQC (version 0.11.5)