FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4084019
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA10400.014.71680451
AACGGGA8800.014.3173448
CGTCCCG2052.1822416E-814.26850635
CGCCATA950.005366370514.2156121
AAACGGG11400.013.6184577
CCTCGAT1555.7973866E-513.0691931
CCCGATC2309.856194E-812.71758238
GAAACGG11750.012.6383326
ACGGGAT7200.012.4992699
CCGATCT2452.2436325E-711.93895439
GGAAACG12400.011.7948215
CGGGATA1953.8513957E-511.537786510
GTCCCGA2553.7693462E-711.47075936
TCTCGTA2758.086863E-811.45541943
ACCCGGC3256.1154424E-911.07708836
ACCGCGC3256.1227183E-911.07627531
TCCCGAT2656.1976425E-711.03790137
CGGGATT6550.010.648231510
CTCGTAT2759.978085E-710.63743444
CCGCAAT1500.0087650310.5029862
Produced by FastQC (version 0.11.5)