FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8179307
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23750.012.5613721
AACGGGT5600.012.0460748
CCGGCTA8550.011.83596938
AAACGGG23750.011.6453117
GCGCGAT3451.3315002E-911.07999220
CGCGATC3653.3742253E-910.47286921
GAAACGG24750.010.4525796
CGGCTAA9050.010.43657939
AACGGGA18950.010.323398
CCCCGAT3103.9740007E-710.2068821
TGGCGCG4253.783498E-1010.05250518
CGCGCCC5151.8189894E-1210.04241633
ACCACGC17650.09.93727931
CGGGATT12450.09.93357610
GGCGCGA3858.107236E-99.92882319
GGAAACG25700.09.8447855
CGTTGCA4651.70985E-109.720841
CGACAAT2109.332128E-49.6861231
CCCCGAA4457.9489837E-109.6498451
ACGGGAT12950.09.3764039
Produced by FastQC (version 0.11.5)