FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13686317
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC384550.2809740560590552TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT49100.036.7484242
TCTCGTA49150.036.7115741
CGTATGC50500.035.9075944
CGCCAAT50750.035.38006633
TATCTCG51100.035.26751339
TCGTATG51750.035.08373343
ATCTCGT52000.034.8725640
ACGCCAA53700.033.51941332
GTCACGC56750.031.79594429
GTATGCC59650.030.36183545
AGTCACG60000.030.07256528
GATCGGA62350.029.326441
TCGGAAG67500.027.2034653
ATCGGAA67500.027.1701262
CGTCTGA67050.027.111616
CACGCCA68850.026.56851431
ACACGTC69300.026.3287513
CGGAAGA70700.026.0344184
ACGTCTG70600.025.74834315
CACGTCT72550.025.2113314
Produced by FastQC (version 0.11.5)