FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6219653
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA7150.028.30799343
CTCGTAT7050.028.07040644
ATCTCGT8100.025.26556242
TCGTATG7800.024.8690845
AACGATC8450.023.95291538
GTCACGT8900.023.4989329
GATCGGA9100.021.8474921
AGTCACG9800.021.57016428
AAACGAT9500.021.54169837
ACGATCT9850.020.54843939
TCACGTG10600.019.94238530
TCGGAAG10400.019.5413973
ATCGGAA10250.019.3866062
ACACGTC10700.019.3348913
CGTCTGA10750.019.03577616
CGTGAAA10900.018.7744233
ACGTGAA11100.018.63888732
TGAAACG11300.018.10954935
CACGTGA11500.017.99057831
CGGAAGA11150.017.821914
Produced by FastQC (version 0.11.5)