FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922261
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.019.2856245
CTCGTAT5500.014.72638541
TCTCGTA5800.014.35258440
CGTATGC6000.013.49993643
ACCGCGC7100.012.67567531
CCGCGCC7300.011.7118632
CGTTGGA25450.011.6785361
GCGTGAG21550.011.37982522
CACCGCG8000.011.2497230
TAGGCGT3856.184564E-1111.10333919
AAACGGG27900.010.8063467
CGTGAGC23800.010.6821323
ACGCCCG4509.094947E-1210.49936734
AACGGGA21850.010.4000938
CGTCTGA9000.010.249536515
ACACGTC9450.010.23773712
CGCGCCC4853.6379788E-1210.20577633
ATAGGCG3755.2150426E-910.19953918
ACGGGAT15800.010.1104069
AACGGGT7350.010.10168658
Produced by FastQC (version 0.11.5)