FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6728137
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA123790.18398852460941267TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14100.027.842851
TCGGAAG14950.026.2951553
ATCGGAA15150.026.2451422
CGTCTGA15200.025.6528516
ACACGTC15300.025.48499513
ACGTCTG15350.025.4021715
GCACACG15900.024.71193511
CGGAAGA15850.024.7051874
CACGTCT16150.024.23671714
CACACGT18250.021.44767612
GAGCACA24050.016.8364819
AGCACAC24400.016.717910
AGAGCAC23650.016.6773578
CAGTCAC24500.016.4677427
GAAGAGC24500.016.349926
GTCACTG25000.016.31848729
TCTGAAC25650.015.84483718
GTCTGAA26800.015.22088617
CCAGTCA26200.015.17023826
AGTCACT27750.015.07957228
Produced by FastQC (version 0.11.5)