FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8072329
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22650.013.2547211
ACGGGAT14700.012.6979499
AACGGGA20150.012.6117748
AAACGGG24900.012.46386057
GAAACGG25550.012.0618936
CGGGATT14300.011.9522710
GGAAACG27500.011.4860825
CGTATGC3053.4014556E-710.32320444
TCGTATG3451.7435923E-79.7782543
AACGGGT5850.09.61074358
CGCCCGG9450.09.28157635
GCCACCG18000.08.87274928
CTCGTAT3552.6950693E-68.86928642
ACCGCGC7000.08.67658631
TCTCGTA4001.478873E-68.43374141
ACGGGTT5352.6666385E-98.4071559
ACAGGCG29650.08.34335718
CCCAAGT41150.08.0088511
CACCGCG7601.8189894E-127.991591530
GATTTCG9950.07.9363635
Produced by FastQC (version 0.11.5)