FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5311301
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC3450.014.99756833
ACCGCGC4250.014.82112631
CACCGCG4450.014.66054330
CGTTGGA17000.014.3217321
CGTATGA1453.0525312E-513.99249651
ACGGGAT10600.013.5824469
CGGGATT9150.013.52214210
AACGGGT3750.013.1976118
AAACGGG18100.012.9282727
CGTGAGC13000.012.63280523
GCCACCG11700.012.30558128
GCGTGAG13250.012.05487622
GAAACGG19250.012.0483516
CCGCGCC5050.012.02775232
AACGGGA15400.011.9783528
GGAAACG19800.011.9425875
ACGGGTT3408.185452E-1111.9096099
GATCGGA3451.0186341E-1011.7618081
CGCCCGG5400.011.66466635
ACGCCCG2901.3558747E-811.636044534
Produced by FastQC (version 0.11.5)