FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6511831
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20000.017.7552191
ACGGGAT13600.017.0296699
AACGGGA16500.016.8984018
CGGGATT13700.015.92058610
AAACGGG21700.014.7141947
GGAAACG22600.014.3818835
GAAACGG23150.013.7010166
TATGCCG2251.0159729E-612.02463645
TGCGCCC3753.8198777E-1111.39646833
GCGTGAG13900.011.32516322
CTACGTC2201.0680944E-511.24366635
ACTGCGC4850.011.13057131
CCCGTAA2301.6393951E-510.8173571
CCCCGAA2759.343239E-710.6921991
CGTGAGC14700.010.40286223
CACTGCG5950.010.20692130
CCCGTTC2005.9206557E-410.1781511
CCCCGAC3154.896974E-710.0524951
GCCACCG12600.09.8181628
ACAGGCG20650.09.800096518
Produced by FastQC (version 0.11.5)