FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588591
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23900.014.1411241
ACGGGAT15500.013.6437549
CGGGATT14650.013.36039610
AACGGGA21000.013.1772128
AAACGGG25300.012.27146157
GAAACGG26500.011.8855676
GGAAACG26500.011.7235545
ACCGCGC5950.010.96525731
ACAGGCG27400.010.34564818
CACCGCG6400.010.194262530
CGTTAAC2006.133756E-410.1391861
CCGCGCC6450.09.76643532
CGCGCCC4651.8735591E-109.67642233
TATGCCG2150.00119964859.4238883
CCTCGTA2403.10628E-49.3881351
GCCACCG17200.09.15603328
GATTTCG10350.09.1355565
CCCGTAA3003.170452E-59.0126091
AGGCGCC7600.08.88066420
GCGTGAG21550.08.66522
Produced by FastQC (version 0.11.5)