FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6788859
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18200.013.0473981
AACGGGA16550.012.0913988
AAACGGG19850.011.8934247
GAAACGG20500.011.2967926
CGTAGAA3255.647962E-911.1337791
ACGGGAT12100.010.9635499
CGGGATT10900.010.93286210
GGAAACG21600.010.6712355
CCCGGAA2851.4808265E-610.3158371
CCCGGTT3103.938676E-710.2134471
CGGGAAT7550.010.125510
CGCACCC4152.6593625E-99.75428233
ACCGCAC5453.6379788E-129.490787531
ACGCCCG3351.2308992E-69.39646634
ACGGGAA8200.09.0486679
CCGGTTT2555.529532E-48.8621232
GATTTCG9650.08.6644845
GCGTGAG16150.08.63222722
CGCCCGG6705.456968E-128.39007335
CGTGAGC17650.08.28086223
Produced by FastQC (version 0.11.5)