FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8010572
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10250.030.94858442
TCTCGTA10800.029.3732341
CGATGTA10950.028.97266834
CGTATGC10900.028.8966244
TCGTATG11000.028.83845343
CCGATGT11200.027.72327633
TATCTCG11400.027.63043239
CACCGAT11800.026.5034731
ATCTCGT12250.026.26372540
ACCGATG12150.025.36963532
GTCACCG13900.023.14652329
TCACCGA14250.022.42012430
GATCGGA16150.020.380551
ATCGGAA17300.019.2501582
GTATGCC17500.018.38403345
TCGGAAG17850.018.2788353
CGTCTGA17950.018.17416616
ACACGTC18350.017.90060613
ACGTCTG19150.017.03531615
CGGAAGA20800.016.009954
Produced by FastQC (version 0.11.5)