FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254047
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22650.013.0515191
AACGGGA20100.012.3878248
AAACGGG24000.012.37488657
GCGTGAG19000.011.84216922
ACGGGAT15800.011.7716989
ACAGGCG24400.011.67987718
CGTGAGC20000.011.62506323
GGAAACG23600.011.5677465
CGGGATT15450.011.4558710
GAAACGG25400.011.2794236
GATCGGA3600.011.2544511
CCCTAAA26200.011.1112641
CCCAAAG85800.010.7385131
GAGCCAC52200.010.4310926
ATCGGAA4750.010.4212612
CGGGAAT7600.010.06540110
CCCGAAT5550.010.0039571
CACTGCG8500.09.88250330
CCCGGAT3203.274181E-119.8476451
CCCAAGT34200.09.3019251
Produced by FastQC (version 0.11.5)