FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8656558
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23950.012.7233651
AACGGGT5900.011.8206448
ACGGGAT15500.011.7566829
AAACGGG25600.011.3368857
GAAACGG25850.011.3142786
CGGGATT15100.011.02519410
GGAAACG26350.010.92882255
AACGGGA21250.010.163518
ACGGGTT5353.6379788E-129.671769
GCGTGAG19300.09.55862722
ACAGGCG29200.09.39968818
ACCGCGC6250.09.35895331
CGCGCCC5557.2759576E-129.32333633
CGTGAGC20800.09.08562723
CCCCGGT3806.7110705E-78.9100751
CCCAAAG89650.08.86271
GCCACCG19950.08.68318928
CACCGCG7050.08.61605830
CGCCACC13250.08.48966627
CTCGTAT2450.0039513538.26447844
Produced by FastQC (version 0.11.5)