FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9371839
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1203261.2839102336265058TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG451680.4819545022060238TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA132550.043.6183741
TATGCCG62400.042.52150745
CGTCACG5150.033.19497727
CGCCAAT186700.030.75869233
ACGCCAA188500.030.45336532
TATCTCG191900.029.83175539
CTCGTAT192550.029.7896242
TCTCGTA192700.029.7896241
ATCGGAA194850.029.689682
AGTCACG193750.029.6281828
CGTATGC194500.029.6066144
TCGTATG194600.029.52204143
ATCTCGT194750.029.42984240
TCGGAAG196700.029.4218983
GTCACGC194650.029.42184829
CGGAAGA200200.029.1776944
ACACGTC198400.029.1260413
CGTCTGA198300.029.106716
ACGTCTG200600.028.795415
GTATGCC199900.028.78896745
Produced by FastQC (version 0.11.5)