FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9762342
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25700.014.0257231
CTCGTAT4750.013.73555544
TCTCGTA4850.013.45207343
TCGTATG5700.013.43038945
ACGGGAT17600.012.781789
AAACGGG28650.012.2490947
AACGGGA24350.012.1025358
CGGGATT16100.012.01621510
GAAACGG29700.011.743556
CCGTATC5950.011.72057138
GGAAACG29450.011.7037965
AGTTCCG6450.010.46317234
GATTTCG12000.010.3252855
TATCTCG7250.010.23968641
TCCGTAT6900.010.10686937
CGGCCGA1800.00296167779.99819239
CGTATGC4102.1136657E-99.88492145
CGTATCT7650.09.41006439
ATCTCGT7250.09.30880542
ACAGGCG32200.08.942318
Produced by FastQC (version 0.11.5)