FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474883
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG198120.3059823629245501TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25400.039.15229444
CCGTATC26150.038.80373438
TCTCGTA25700.038.6077243
AGTTCCG26800.038.1141634
TATCTCG26250.037.88450641
TCGTATG26250.037.62737345
GATCGGA26300.037.5056231
TCCGTAT27350.037.26543437
CGTATCT27150.037.12588539
TTCCGTA28100.036.35087236
ATCTCGT27400.036.29446442
GTTCCGT29500.034.9308235
ATCGGAA28550.034.5998342
ACACGTC30900.033.63970613
CGTCTGA31050.033.40447216
TCGGAAG29500.033.256773
CGGAAGA30500.032.1663864
CACGTCT33250.031.60049414
ACGTCTG33050.031.38326515
GCACACG34100.030.41715211
Produced by FastQC (version 0.11.5)