FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7118416
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4150.017.88913242
TCTCGTA5250.014.56944541
AAACGGG22200.014.0875047
CGTTGGA22950.013.8469371
TCGTATG5250.013.71241943
CGTATGC5700.013.41922744
GAAACGG23750.013.1741246
AACGGGT5500.013.0885758
AACGGGA18300.012.9075368
GGAAACG23350.012.8213065
ATCTCGT5800.012.79971140
ACGGGTT5100.012.7923769
CGGGATT13450.012.71202410
ACGGGAT14200.012.5159029
TCGGATA1506.4536993E-412.00055929
CGCGCCC4500.011.49827333
ACCGCGC5400.010.41510331
CGTGAGC18450.010.12270723
CATCTCG7150.010.06834739
GCGTGAG17950.010.02691322
Produced by FastQC (version 0.11.5)