FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445667
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG450.00122824.99709519
CGTTGGA23400.016.9807821
ACGGGAT15950.016.2206869
CGGGATT15200.015.68896710
AAACGGG24600.015.3640687
AACGGGA20600.015.2894868
GAAACGG24950.014.6075226
GGAAACG25750.013.8915895
ACCGCGC4850.013.45235731
CGCGCCC3501.0913936E-1112.21305633
CACCGCG4850.012.0606430
AACGGGT4900.011.9373898
CCGCGCC5150.011.79511332
ACGCTAG2056.4107975E-510.97527127
GCGTGAG15900.010.75346822
CGCTAGA1903.8732213E-410.65756628
GCTAGAC13750.010.63603529
CCCGTAA2453.3595017E-510.1364871
CGTGAGC16700.010.104321523
GTTGGAA42750.010.0560662
Produced by FastQC (version 0.11.5)