FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12383221
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7450.027.18091645
CTCGTAT11000.018.6147941
TCTCGTA11050.018.12424740
CGTATGC11350.017.6429143
GATCGGA5900.017.5528871
TATCTCG11700.016.92521938
ATCTCGT12300.016.28254939
CGTCTGA12650.015.649034515
ACACGTC13300.015.39295412
TCGTATG13250.015.28279242
ATCGGAA13650.014.8440571
TCGGAAG14250.014.2134152
ACGTCTG14600.013.71399914
CGTTGGA36900.012.995591
ACGGGAT23150.012.4392659
CGGAAGA16600.012.3334583
CACGTCT16700.012.25891113
AAACGGG38750.011.7269177
AACGGGA33050.011.6401998
CGGGATT20600.011.57642310
Produced by FastQC (version 0.11.5)