FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5671368
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA7650.033.79872541
CGTATGC8400.031.58062244
CTCGTAT8250.031.3373242
TATCTCG8400.030.78043739
ATCTCGT8700.029.46061540
TCGTATG9250.029.40773843
GATCGGA8750.029.0000611
ACACGTC10450.026.03031213
CGTCTGA10650.025.75256316
ATCGGAA10250.025.6298222
TCGGAAG10300.024.8457813
CACGTCT11850.023.33442114
ACGTCTG11900.023.23637815
GTATGCC12150.022.77291945
CGGAAGA11750.022.3567394
GCACACG13950.019.82171411
CACTAGC13750.019.45604531
CACACGT14550.018.84981512
GCTTATC14600.017.555336
ACTAGCT15700.017.18271832
Produced by FastQC (version 0.11.5)