FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297412
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG5800.012.79707830
ACCGCGC5250.012.4240631
ACGGGAT13200.011.75672159
CGCCCGG6000.011.62271935
AAACGGG22900.011.589317
CGTTGGA23150.011.3985871
AACGGGA19600.011.360318
CCGCGCC5750.011.34406832
CGCGCCC3804.9112714E-1111.24609633
GCCCGGC11850.011.20033336
GAAACGG23500.011.1977066
GCCACCG13450.011.03697328
GGAAACG23250.010.9592735
GCGCCCG4950.010.90677634
CGGGATT12500.010.615808510
GCGTGAG14900.010.56638122
TCTCGTA3002.7031456E-710.49710743
GGCGCAA2006.2317285E-410.12164719
TGCCCGG7100.09.82201635
CGGGAAT8950.09.80059910
Produced by FastQC (version 0.11.5)