FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9906740
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA302040.3048833420479391TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA34850.026.8714431
ACACGTC35400.026.35398913
TCGGAAG35650.026.3437183
CGTCTGA35400.026.31188616
ATCGGAA35900.026.125472
ACGTCTG36350.025.62423115
CACGTCT36650.025.41422714
CGGAAGA37100.025.354554
GCACACG37800.024.7997611
CACACGT39900.023.53209912
AGAGCAC47550.019.840918
GAAGAGC49950.019.1883566
CAGTCAC49650.019.1836527
GAGCACA49450.019.078579
TCTGAAC49800.018.91446118
AGCACAC50850.018.84826310
GTCACTG51650.018.52812829
AGTCACT52550.018.03954328
CCAGTCA52900.017.91991626
GTCTGAA53350.017.65585917
Produced by FastQC (version 0.11.5)