FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9965761
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8250.022.08991443
CTCGTAT8400.021.6954544
TCGTATG8550.021.31504445
ATCTCGT10450.018.30061142
AATCTCG14250.013.104671541
ACCGCGC6950.012.94918631
CGTTGGA25700.012.8937831
AAACGGG27050.012.6423037
AACGGGA22150.012.3917838
CACCGCG7600.011.5456530
ACGGGAT15950.011.4254169
GAAACGG28900.011.2890996
GGAAACG29600.010.5660925
GATTTCG11450.010.4150575
GCGTGAG25700.010.32991122
CGGGATT15150.010.24711410
CCCGGAT4001.3515091E-910.1439861
CGGGAAT10550.010.02329510
CGCCCGG10550.010.02314535
GCCACCG21400.09.67255928
Produced by FastQC (version 0.11.5)