FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521264
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2001.5535988E-814.6417545
CGTTGGA12450.014.1402541
CGCGCCT1758.2254155E-714.13650733
ACGGGAT8350.013.4667069
AACGGGA10450.013.1283658
CGTATGC2602.6830094E-912.97666343
GAAACGG12900.012.7281086
TCTCGTA2502.16005E-812.59573640
AAACGGG12800.012.3001157
CGGGATT7500.011.99434710
CTCGTAT2452.2538552E-711.93486641
GGAAACG14100.011.3631545
TCGTATG2656.225655E-711.03412342
CGCAAGA1450.006845865410.85863344
ACGCCCG2301.7455006E-510.75603934
ACAGGCG15600.09.80307218
CCGCACC3704.627691E-89.72536132
CGTGAGC11950.09.41062423
GCGTGAG12700.09.38544522
ATCTCGT2952.6538657E-59.14944539
Produced by FastQC (version 0.11.5)