FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245599
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG272980.5203981470943547TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34900.040.28938745
CTCGTAT35100.039.996144
CCGTATC35100.039.80684738
TCTCGTA35350.039.77688643
AGTTCCG35600.039.3760434
ATCTCGT35600.039.37116242
TCCGTAT35900.039.10967337
TATCTCG35850.039.0977341
CGTATCT36300.038.8000939
TTCCGTA36350.038.56360636
GATCGGA36900.038.5630231
GTTCCGT36500.038.34348335
ATCGGAA38750.036.7012442
TCGGAAG39700.035.9363673
ACACGTC39900.035.69235213
CGTCTGA39950.035.59136616
CGGAAGA40650.035.2055634
ACGTCTG40850.034.8072215
CACGTCT41650.034.08463714
GCACACG42650.033.4440411
Produced by FastQC (version 0.11.5)