FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6699216
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21450.013.8802931
AACGGGA17700.013.6003238
ACGGGAT13200.013.1238729
AAACGGG20700.012.9334787
GAAACGG21900.012.228816
CGGGATT13250.012.22536410
CGGGAAT7200.011.56150610
GGAAACG24000.011.2522245
CCCGTAA2709.8405944E-59.1892681
GTTGGAA40550.08.7142952
ACAGGCG23900.08.66105518
GCGTGAG16550.08.15706822
ACGGGAA8300.08.1318329
TAGACGG2500.00474113378.09894233
CGTGAGC16700.08.08380123
CGTAGAA3401.3530225E-47.96075631
CCCGAAA2600.00658775547.8076651
CCCTATT15900.07.66035031
CCCAAAA36800.07.47762971
GAGCCAC47850.07.475511626
Produced by FastQC (version 0.11.5)