FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10028094
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT9350.026.2233
CTCGTAT9900.024.535942
GATCGGA10400.023.646241
TATCTCG10550.023.45058439
TCTCGTA10600.023.12790141
ACGCCAA10850.022.3878232
CGTATGC10750.022.38685444
GTCACGC11100.022.28884129
TCGTATG11050.022.1859343
ATCTCGT11800.020.77580840
ACACGTC12700.020.01169413
TCGGAAG12800.019.917623
AGTCACG12850.019.25349228
ATCGGAA13000.019.0905422
CGTCTGA13300.018.93980416
CGGAAGA14500.017.7380454
ACGTCTG15100.016.6820815
CACGTCT15500.016.39667714
GCACACG17050.015.30180711
CGTTGGA27550.014.24941
Produced by FastQC (version 0.11.5)