FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5369277
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG167820.3125560480489272TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21450.040.788744
TATCTCG21450.040.68384641
TCTCGTA21750.040.226143
GATCGGA21950.039.6811371
GTCACGT22400.039.05845629
TCGTATG22550.038.77220545
ATCGGAA22500.038.5895962
ATCTCGT22800.038.57087342
AGTCACG22950.038.4164228
ACACGTC23400.037.96599613
ACGTGGC23350.037.4752932
TCGGAAG23650.036.8085023
CGTCTGA23850.036.7781416
CGTGGCC24150.036.3270233
CACGTCT24500.036.16959414
ACGTCTG24400.036.041315
CGGAAGA24850.035.4848024
TCACGTG25150.034.7037230
GCACACG25750.034.67582711
CACACGT27000.033.0704612
Produced by FastQC (version 0.11.5)