FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11181982
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2001.2914825E-1012.7518571
CGTTGGA28750.012.41921
AACGGGA24150.011.6765268
ACGGGAT17800.011.3759249
GAAACGG30350.011.317486
AAACGGG31000.011.2737197
CACCGCG7700.010.90936330
GGAAACG30600.010.882045
GCCACCG17700.010.42408228
CGGGATT19150.010.18233810
AACGGGT6350.09.9208418
ATAGGCG3051.364242E-109.8356518
GCGTGAG20000.09.82528922
CGTAGTA1552.286821E-49.6788291
ACAGGCG28650.09.63310118
CGTGAGC20950.09.37979323
GAGCCAC58150.09.18337526
CCACCGC15600.09.13492429
CCCAAAG92050.08.8986341
CGGGAAT10250.08.48744810
Produced by FastQC (version 0.11.5)