FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11488983
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6150.027.80426645
CTCGTAT7550.022.64890341
CGTATGC7850.021.49643543
TCTCGTA7850.021.2140
TCGTATG9500.018.2365242
ATCTCGT9800.017.6784939
CGTCTGA11900.014.93573615
ACACGTC12250.013.77442612
TCGGAAG12450.013.73852352
ATCGGAA12500.013.6887551
CGTTGGA33150.013.6512641
ACGTCTG13400.013.432013514
AATCTCG14000.013.01779738
AACGGGA27700.012.7529278
ACGGGAT20700.012.60891159
GTATGCC14400.012.1880844
AAACGGG34100.012.0747247
CACGTCT14750.011.59230813
CGGAAGA14850.011.5175543
CGGGATT20300.011.41635210
Produced by FastQC (version 0.11.5)