FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6877996
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21200.013.4115261
AAACGGG20950.012.8827387
AACGGGA18200.012.4813328
GAAACGG22700.011.9906616
CGGGATT12600.011.95955910
ACGGGAT13200.011.7567199
GGAAACG23850.011.5376835
CCCGATT2553.632358E-711.5039421
CGCCCGG7400.010.02996635
GCGTGAG16000.09.41822222
CGGGAAT8900.09.35023810
ACGCCCG4651.9190338E-99.19038534
CACCGCG4905.0749804E-109.18051330
CCGGCTA7650.09.11417838
ACGGGAA8400.09.1035459
ACCGCGC4702.3283064E-99.09261531
CGTGAGC17100.08.68103323
CCCCGTT3451.7274337E-58.5029131
CCCGTAA3206.641709E-58.4620341
ACAGGCG23100.08.2759518
Produced by FastQC (version 0.11.5)