FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241158
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2150.016.04167713
GATCGGA2150.015.3694551
ATCGGAA2550.014.1324162
CACCGCG3950.012.93101930
CGTTGGA14850.012.6432241
CCCCGAA2401.8189894E-1212.5167911
CCCGAAC1101.0341817E-412.2855652
ACGTCTG2800.011.78216515
TCGGAAG2701.8189894E-1211.6784963
CGTCTGA3050.011.30806816
CCCAACG1501.3417928E-511.0147761
AACGGGA13600.010.6953088
GCCACCG9600.010.46559628
CCCCGAT1304.8603467E-410.3985651
AAACGGG17350.010.3715147
ACGGGAT9750.010.3045879
CGGGATT9300.010.1582310
CGATAAA2105.1651296E-710.0134331
CACGTCT3152.3646862E-119.99698814
GAAACGG18200.09.972486
Produced by FastQC (version 0.11.5)