FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8469070
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG2700.012.77710318
CGTTGGA23100.012.6003081
ACGGGAT14350.012.4383699
AACGGGA20150.011.4634158
GAAACGG26100.011.4361796
AAACGGG25500.011.4111637
CGGGATT14350.011.39312710
GGAAACG24350.011.2726395
GCGTGAG13800.010.32645622
CGTGAGC14500.010.13829523
GCCACCG12900.09.88390128
AACGGGT5200.09.5187288
CCCAAAG68050.09.5025591
CCTACGG2403.2049502E-79.3771641
CCACCGT7050.09.36192729
CCACGCG1300.00571563379.2328991
CCCTAAA20550.09.0531831
GATCGGA1500.00182137599.0020761
AAGCGGC5500.09.0003763
CGGGATA2505.8099795E-78.99952510
Produced by FastQC (version 0.11.5)