FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529454
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4200.025.17647745
CTCGTAT4500.023.49804743
TCTCGTA4600.022.98689342
TCGTATG5450.018.57643744
CGTCACG851.2059754E-418.52813327
AACGATC5800.018.2317637
ATCTCGT5950.018.14949441
AAACGAT6800.015.55061936
GTCACGT7350.014.99896628
TGAAACG7150.014.789434
ACGATCT6750.014.66586438
ACCGCGC3101.8189894E-1213.789371531
GAAACGA7850.013.75720835
CACCGCG3450.013.69470830
CGTGAAA8200.013.44438532
AGTCACG7350.013.16235827
TATGCCG1200.001655799613.1268843
ACGTGAA8400.013.12409531
CACGTGA8250.013.09000730
TCACGTG8300.013.01115129
Produced by FastQC (version 0.11.5)