FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7717513
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA20100.013.873318
CGTTGGA23350.013.5474431
AAACGGG24200.013.4744157
ACGGGAT14650.012.8942529
TCGTTCG1250.00220054812.6399824
GAAACGG25150.012.6102266
CGGGATT13850.012.50245910
GGAAACG25500.012.0381575
ATCGCGC2201.0674825E-511.24431337
GATTTCG9350.010.6219035
CGGGAAT9250.010.45396910
TCGCGCC2201.3182874E-410.22210238
GATCGCG2657.461418E-610.18352936
CGTTGGT3552.4686233E-79.5472681
GCGTGAG20200.09.351706522
ACGGGAA10100.09.3515249
CGTGAGC19700.09.13261523
CGAAACG2000.00708107238.99527559
GTTCGAA3552.69058E-68.8703636
CGTGCCC7150.08.80690633
Produced by FastQC (version 0.11.5)