FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527872
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC7050.030.31818633
ACGTCCG7200.029.99904832
TCTCGTA7350.029.69313443
CTCGTAT7500.029.39926344
TCGTATG7950.027.45195845
GTCCGCA7850.027.22843434
TCCGCAC8300.026.02327235
CGCACAT9200.023.96663337
ATCTCGT9250.023.59400442
CATCTCG10200.021.83768841
CCGCACA10200.021.83754236
CACGTCC10700.020.3965531
GATCGGA9950.020.3712731
AGTCACG11100.019.66153728
GTCACGT11200.019.48598929
ATCGGAA10450.019.3794362
TCACGTC11700.019.23003230
TCGGAAG11350.018.2392443
ACACGTC11850.018.03728313
CGTCTGA12900.016.56913216
Produced by FastQC (version 0.11.5)