FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6779872
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16200.012.6023791
CCCCGAT1459.2687205E-611.3881531
AACGGGA14350.011.2870568
AAACGGG17700.011.2690477
GAAACGG17950.011.2001066
ACGGGAT10700.011.0726649
GGAAACG18700.010.755195
CGGGATT9950.010.249310510
CCCAAGT23300.09.9218671
CCCAAAG52850.09.2313871
ACAGGCG16850.09.07839618
CACCGCG5200.08.94648330
GCGTGAG12600.08.92687722
GTTCGAG7700.08.7645714
GAGCCAC33650.08.64620426
AACGGGT3851.4188117E-108.5698018
CGTGAGC13150.08.5535123
CCCTAAA16450.08.3955771
CCCGAGT9800.08.27172851
CCCGAAT4003.0559022E-108.2564111