FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8693835
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATC800.001648612813.1348291
CGTTGGA21850.012.2975321
AACGGGA18500.011.8371658
AAACGGG22950.011.6331247
CGGGATT14150.011.34211110
CCCGATT2155.3605618E-911.171151
ACGGGAT14900.010.9725299
GAAACGG24000.010.8741946
GGAAACG23350.010.7298485
CGTCCTA1408.125405E-510.7223091
ACAGGCG15950.010.62623118
CACCGCG4550.010.54891130
CGTCGAA1000.00873625610.5078621
GCGTGAG11900.010.33542622
CGTGAGC11550.010.12917723
CCCTAAA21950.09.5743621
CCCAAAG63850.09.0043891
GGCGTGA14600.08.73227921
GAGCCAC36700.08.705276526
GGATTAC52850.08.68451313
Produced by FastQC (version 0.11.5)